Plant tissues present intriguing systems for study by mass spectrometry imaging, as they exhibit a complex metabolism and a high degree of spatial localization. This chapter presents a methodology for preparation of plant tissue sections for matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) analysis and the use of a hybrid mass spectrometer for “multiplex” imaging. The multiplex method described here provides a wide range of analytical information, including high-resolution, accurate mass imaging and tandem MS scans for structural information, all within a single experiment. While this procedure was developed for plant tissues, it can be readily adapted for analysis of other sample types.
CITATION STYLE
Korte, A. R., Yagnik, G. B., Feenstra, A. D., & Lee, Y. J. (2015). Multiplex MALDI-MS imaging of plant metabolites using a hybrid MS system. Methods in Molecular Biology, 1203, 49–62. https://doi.org/10.1007/978-1-4939-1357-2_6
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