Accumulating evidence implicates phosphoinositide 4-phosphate as a regulatory molecule in its own right recruiting specific effector proteins to cellular membranes. Here, we describe biochemical and immunocytochemical methods to evaluate tetraspanin-associated phosphoinositide-4 kinases activity in primary human hepatic stellate cells (hHSC) and neoplastic hepatoblastoma cells.
CITATION STYLE
Rombouts, K., & Carloni, V. (2016). Determination and characterization of tetraspanin- Associated phosphoinositide-4 kinases in primary and neoplastic liver cells. In Methods in Molecular Biology (Vol. 1376, pp. 203–212). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3170-5_17
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