Abstract
Validation of a mouse model of dengue virus (DENV) infection relies on verification of viremia and productive replication in mouse tissues following infection. Here, we describe a quantitative assay for determining viral RNA levels in mouse serum and tissues. For the purpose of confirming DENV replication, we outline a fluorescence immunohistochemistry (FIHC) protocol for staining a nonstructural protein of DENV.
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APA
Plummer, E., & Shresta, S. (2014). Animal models in dengue. Methods in Molecular Biology, 1138, 377–390. https://doi.org/10.1007/978-1-4939-0348-1_23
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