Elimination of error caused by hemolysis and bilirubin induced color quenching in clinical radioimmunoassays

Abstract

In radioimmunoassays and radioassays for various constituents of serum, color quenching is a source of error when liquid scintillation methods are used in samples that contain hemolyzed blood and samples from jaundiced patients. A quench correction can be made by using the External Standard Channels Ratio method and normalizing the ratios, or alternatively, by chemical decolorizartion with tetramethylammonium hydroxide, hydrogen peroxide and ascorbic acid.