Electrochemical behavior of anticancer drug 5-fluorouracil at carbon paste electrode and its analytical application

Abstract

Background: A set of pyrimidine nucleobase present in all living systems as a component of nucleic acid constitutes uracil together with thymine and cytosine. A diverse physiological activity is exhibited by many N-substituted uracil derivatives. In oncology, 5-FU is widely used as an important anticancer drug. Methods: Electrochemical behavior was studied using cyclic voltammetric method, and the analytical application was studied using differential pulse voltammetric method. Solution pH has been measured by pH meter. Results: The process on the surface of electrode was found to be irreversible and diffusion controlled. The charge transfer coefficient, heterogeneous rate constant, and the number of electron transferred were calculated. Possible reaction mechanism taking place on the surface of electrode was proposed. Calibration plot constructed using differential pulse voltammetric technique was used for quantitative analysis in pharmaceutical and human urine sample. Limit of detection (LOD) and limit of quantification (LOQ) were calculated to be 12.25 and 40.8 nM, respectively. Conclusions: In the present work, we described the electrochemical behavior of anticancer drug and its determination in human urine and pharmaceutical samples. The method shows the development of a sensor for selective and sensitive determination of 5-FU.