ISOLATION AND PROPERTIES OF AN EXOCELLULAR NUCLEASE OF SERRATIA MARCESCENS

Abstract

Eaves, George N. (Wayne State University College of Medicine, Detroit, Mich.) and Charles D. Jeffries . Isolation and properties of an exocellular nuclease of Serratia marcescens . J. Bacteriol. 85: 273–278. 1963.—The exocellular nuclease of Serratia marcescens , isolated by anion-exchange chromatography on diethylaminoethyl-Sephadex, depolymerized deoxyribonucleic acid, ribonucleic acid, and the polynucleotide which is refractory to pancreatic ribonuclease activity. The enzyme was tentatively classified as a nonspecific phosphodiesterase. Magnesium was essential for activity, which was optimal at pH 8.8. The purified enzyme was completely inactivated by heating at 50 C for 15 min.