HPTLC METHOD FOR THE DETERMINATION OF LUPEOL FROM ANDROGRAPHIS ECHIOIDES LEAVES

Abstract

Objective: A new simple, precise, rapid and selective high‐performance thin‐layer chromatographic (HPTLC) method has been developed for the determination of lupeol in methanolic leaves extract of Andrographis echioides.Methods: As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of lupeol as standard on HPTLC plates for the quantification of lupeol from the Andrographis echioides leaves. The concentration of standard lupeol was 1 mg/ml.Results: The retention factor of lupeol was found to be 0.55. Linearity was obtained in the range of 5000 ng‐10000 ng for lupeol. The developed and validated HPTLC method was employed for lupeol in methanolic leaves extract of Andrographis echioides for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99917 for lupeol. Satisfactory recoveries of 99.80 % were obtained for lupeol.Conclusion: The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of lupeol in methanolic leaves extract of Andrographis echioides.