Two methods that use chemoselective ligation chemistry to prepare peptide and small-molecule microarrays are described here. The first method involves the functionalization of a glass slide with a glyoxylyl group, followed by chemoselective ligation of small molecules or peptides to the functionalized surface via a covalent bond. In the second method, peptides or small molecules are first conjugated to a macromolecular scaffold. The final ligand-scaffold conjugates are then spotted and adsorbed onto the solid surface. Three different assay methods to screen such chemical microarrays are described.
CITATION STYLE
Marik, J., & Lam, K. S. (2005). Peptide and small-molecule microarrays. Methods in Molecular Biology (Clifton, N.J.), 310, 217–226. https://doi.org/10.1007/978-1-59259-948-6_15
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