Dose‐dependent beneficial effects of tryptophan and its derived metabolites on akkermansia in vitro: A preliminary prospective study

Abstract

Akkermansia muciniphila, a potential probiotic, has been proven to lessen the effects of sev-eral diseases. As established, the relative abundance of Akkermansia is positively correlated with tryptophan metabolism. However, the reciprocal interaction between tryptophan and Akkemansia is still unclear. Herein, for the first time, the possible effects of tryptophan and its derived metabolites on A. muciniphila were preliminarily investigated, including growth, physiological function, and metabolism. Obtained results suggested that 0.4 g/L of tryptophan treatment could significantly promote the growth of A. muciniphila. Notably, when grown in BHI with 0.8 g/L of tryptophan, the hydrophobicity and adhesion of A. muciniphila were significantly improved, potentially due to the increase in the rate of cell division. Furthermore, A. muciniphila metabolized tryptophan to indole, indole‐3‐acetic acid, indole‐3‐carboxaldehyde, and indole‐3‐lactic acid. Indoles produced by gut mi-crobiota could significantly promote the growth of A. muciniphila. These results could provide a valuable reference for future research on the relationship between tryptophan metabolism and A. muciniphila.