The CRISPR/Cas system is a highly specific genome editing tool capable of distinguishing alleles differing by even a single base pair. Target sites might carry genetic variations that are not distinguishable by sgRNA designing tools based on one reference genome. AlleleAnalyzer is an open-source software that incorporates single-nucleotide variants and short insertions and deletions to design sgRNAs for precisely editing 1 or multiple haplotypes of a sequenced genome, currently supporting 11 Cas proteins. It also leverages patterns of shared genetic variation to optimize sgRNA design for different human populations. AlleleAnalyzer is available at https://github.com/keoughkath/AlleleAnalyzer.
CITATION STYLE
Keough, K. C., Lyalina, S., Olvera, M. P., Whalen, S., Conklin, B. R., & Pollard, K. S. (2019). AlleleAnalyzer: A tool for personalized and allele-specific sgRNA design. Genome Biology, 20(1). https://doi.org/10.1186/s13059-019-1783-3
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