Effectiveness of the binahong leaf extract (Anredera cordifolia) in devoting bacterial growth Vibrio cholerae in vitro

Abstract

Parameters that determine biological water quality are microbial parameters of pollutants, pathogens and toxin producers. Many microbes often mix with water, especially in shallow groundwater. One of the antioxidant-producing plants is the Binahong plant which has a high enough antioxidant level which inhibits bacterial growth. The purpose of this study was to determine whether binahong leaf extract can inhibit the growth of Vibrio cholerae bacteria. This study was a laboratory experimental study using a completely randomized design (CRD) with five treatments. The treatment was repeated 3 times with various concentrations of 30%, 40%, 50%, and 100%, and chlorine was used as a positive contract. The material used in this study was binahong leaf extract macerated with ethanol solvent, a thick extract was produced which was then tested for antibacterial activity on Vibrio cholerae bacteria. The parameters observed were the diameter of the inhibition zone of the growth of Vibrio cholerae bacteria. The result is known that binahong leaf extract has the ability to inhibit the growth of Vibrio cholerae bacteria seen by the presence of inhibitory zones formed. The most effective concentration to inhibit the growth of Vibrio cholerae bacteria was at a concentration of 100% at 6.10 mm at 24 hours of observation.