Trichome Specific Expression: Promoters and Their Applications

Abstract

As often reminded to the readers in articles or reviews which deal with plant adaptation to their environment, higher plants are sessile organisms, a life habit which does not allow them to escape danger or to move to avoid adverse conditions. This environmental pressure has led to a myriad of adaptations, which are reflected in the vast diversity of plant habitats, morphologies, life cycles and physiological adaptations among others. The surface of the aerial parts of plants is a major interaction domain between the plant and its environment and as such is the site of many adaptations, be they chemical or anatomical. Among those adaptations, the leaf hairs or trichomes, which cover the surface of a large number of plant species, play a prominent role. Plant trichomes constitute a world of their own, so great is their diversity. In a review published in 1978 and entitled “A glossary of plant hair terminology”, Payne compiles a comprehensive list of more than 490 terms used to describe trichome morphology (Payne, 1978). Despite this extensive diversity, two major classes of trichome may be distinguished on the basis of their capacity to produce and secrete or store significant quantities of secondary metabolites, namely glandular or non-glandular. Nonglandular trichomes, or leaf hairs, are poorly metabolically active and provide protection mainly through physical means, for example by restricting access to insects, but also by preventing water losses, or protecting against UV radiation. Arabidopsis thaliana has been a model for the study of non-glandular trichome development and many genes involved in non-glandular trichome initiation and development could be identified and characterized (Uhrig and Hulskamp, 2010). The metabolic activity of these non-glandular trichomes is however fairly limited and offers little potential for metabolic engineering. A particular class of hairs is the fibers which are present in various species. Cotton seed trichomes are the most economically important since they are the basis of the cotton fiber, but other species such as cottonwood also have fiber hairs. Glandular trichomes are present in many different plant families and can also be divided in two main classes. The capitate trichomes typically have 1 to 10 glandular cells located at the tip of the trichome stalk, and the secretion is directly exuded from the top cells. The secreted material is in general fairly viscous, and in many cases it makes the leaves sticky. Those trichomes are encountered for example in the Solanaceae (tobacco, tomato, potato, etc.) and in some Lamiaceae species (e.g. Salvia). Peltate trichomes have the capacity to synthesize and store volatile compounds (monoand sesquiterpenes, phenylpropenes) in a subcuticular cavity. Typical representative examples