When does blood haemolyse?: A temperature study

Abstract

Incubation of blood in vitro for up to 1 hour at temperatures below 45°C caused no significant haemolysis as determined by a colorimetric method. The duration of storage of blood in CPD or ACD did not affect the sensitivity of the red cells to haemolysis at 50°C. Blood stored in ACD haemolyses less rapidly at 50°C than blood stored in CPD: but there is no significant difference in the rate of haemolysis at 45°C and below. © 1974 John Sherratt and Son Ltd.