Preimplantation genetic diagnosis - An overview

74Citations
Citations of this article
67Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Since the early 1990s, preimplantation genetic diagnosis (PGD) has been expanding in scope and applications. Selection of female embryos to avoid X-linked disease was carried out first by polymerase chain reaction, then by fluorescence in situ hybridization (FISH), and an ever-increasing number of tests for monogenic diseases have been developed. Couples with chromosome rearrangements such as Robertsonian and reciprocal translocations form a large referral group for most PGD centers and present a special challenge, due to the large number of genetically unbalanced embryos generated by meiotic segregation. Early protocols used blastomeres biopsied from cleavage-stage embryos; testing of first and second polar bodies is now a routine alternative, and blastocyst biopsy can also be used. More recently, the technology has been harnessed to provide PGD-AS, or aneuploidy screening. FISH probes specific for chromosomes commonly found to be aneuploid in early pregnancy loss are used to test blastomeres for aneuploidy, with the aim of replacing euploid embryos and increasing pregnancy rates in groups of women who have poor IVF success rates. More recent application of PGD to areas such as HLA typing and social sex selection have stoked public controversy and concern, while provoking interesting ethical debates and keeping PGD firmly in the public eye. © The Histochemical Society, Inc.

References Powered by Scopus

Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification

1396Citations
N/AReaders
Get full text

Comprehensive chromosomal analysis of human preimplantation embryos using whole genome amplification and single cell comparative genomic hybridization

420Citations
N/AReaders
Get full text

Preimplantation diagnosis for fanconi anemia combined with hla matching

342Citations
N/AReaders
Get full text

Cited by Powered by Scopus

Proof of principle and first cases using preimplantation genetic haplotyping - A paradigm shift for embryo diagnosis

127Citations
N/AReaders
Get full text

Cancer genetic testing and assisted reproduction

94Citations
N/AReaders
Get full text

PGD patients' and providers' attitudes to the use and regulation of preimplantation genetic diagnosis

78Citations
N/AReaders
Get full text

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Cite

CITATION STYLE

APA

Ogilvie, C. M., Braude, P. R., & Scriven, P. N. (2005). Preimplantation genetic diagnosis - An overview. In Journal of Histochemistry and Cytochemistry (Vol. 53, pp. 255–260). https://doi.org/10.1369/jhc.4B6395.2005

Readers over time

‘10‘11‘12‘13‘14‘15‘16‘17‘18‘19‘20‘21‘22‘23‘24036912

Readers' Seniority

Tooltip

PhD / Post grad / Masters / Doc 26

70%

Professor / Associate Prof. 4

11%

Lecturer / Post doc 4

11%

Researcher 3

8%

Readers' Discipline

Tooltip

Agricultural and Biological Sciences 20

40%

Medicine and Dentistry 14

28%

Biochemistry, Genetics and Molecular Bi... 14

28%

Chemistry 2

4%

Save time finding and organizing research with Mendeley

Sign up for free
0