Improved cordycepin production by Cordyceps militaris KYL05 using casein hydrolysate in submerged conditions

Abstract

Cordycepin, a beneficial bioactive product specifically found in Cordyceps, has received attention in various bioindustrial applications such as in pharmaceuticals, functional foods, and cosmetics, due to its significant functions. However, lowproductivity of cordycepin is a barrier to commercialization. in this study, Cordyceps militaris was mutated by UV irradiation to improve the cordycepin production. the highest producer KYL05 strain was finally selected and its cordycepin production was increased about 1.5-fold compared to wild type. in addition, the effects of culture conditions were fundamentally investigated. Optimal conditions were as follows: PH 6, temperature of 25°C, shaking speed of 150 rpm, andculture time of 6days. Effects ofmediumcomponent on cordycepinproductionwere also investigated by using various carbon and nitrogen sources. It was found that glucose and casein hydrolysate (CH) weremost effective as carbon and nitrogen sources in cordycepin production (2.3-fold improvement)with maximum cordycepin production of about 445 mg/L. in particular, production was significantly affected by CH. These results should be of value in improving the efficiency of mass production of cordycepin.