Spatial organization of the genome modulates pivotal biological processes. The emerging new technologies have provided novel insights into genome structure and its role in regulating cell activities. To examine the genome-wide chromatin interactions at accessible chromatin regions, we developed a DNA transposase-mediated analysis of chromatin looping (Trac-looping) method for simultaneously detecting chromatin interactions and chromatin accessibility. Here, we describe a detailed protocol of generating Trac-looping libraries.
CITATION STYLE
Liu, S., Tang, Q., & Zhao, K. (2023). Analysis of Chromatin Interaction and Accessibility by Trac-Looping. In Methods in Molecular Biology (Vol. 2611, pp. 85–97). Humana Press Inc. https://doi.org/10.1007/978-1-0716-2899-7_7
Mendeley helps you to discover research relevant for your work.