A new approach for reagent Storage-Releasing on centrifugal microfluidic platforms using bubblewrap and latex membrane

Abstract

The implementation of latex membranes on centrifugal microfluidic platform has led to the development of advanced liquid handling features, with the exception of a proper reagent storage-releasing technique. This paper presents a new approach for reagent storage-releasing technique using bubbles of a bubblewrap with latex membrane tabs. The bubble was first filled with a liquid sample, sealed with a biocompatible paraffin wax, and then was placed in a chamber of the microfluidic disc. The chamber was then covered with a latex membrane tab similar to single microballoon pump. To release the stored liquid the membrane was manually deflected into the chamber to burst the bubble. The sealed bubbles are leak-free up to 106 kPa, and the average reagent recovery rate is 92%.