Effects and Quantıty Ranges of Some Auxıns on Embryogenıc Callus Inductıon From Upland Rıce Cultıvars: An Overview

Abstract

Embryogenic callus induction from indica rice sub-species, upland cultivars has remain a challenging task, but PGRs have been found to play a vital role in enhancing the production. Hence, solving this tricky problem and achieving optimum embryogenic callus induction from this special type of rice is a welcome development as food security concern, while proper examination of the appropriate PGRs is the only solution. Here we reviewed on two auxins-PGRs that promote the embryogenic callus induction from upland rice, viz., 2,4-D and NAA with their recommendable quantity ranges. 2,4-D and NAA were the most commonly employed and discovered best growth hormones in PTC irrespective of explant in all cereal plants. They are found the most suitable hormones and positively upgrade the production of reproducible callus. Classical analyses implies that medium supplemented with such PGRs provides necessities for quality callus from upland rice. Evidences indicated that callus induction or somatic embryogenesis from matured seeds of upland rice is persuade by 2,4-D between 1.0 – 2.5 mg/L concentrations. Whereas for NAA, to obtain its desired effects on upland rice callus induction it must be fortified in concentrations ranging from 0.5 mg/L to 10 mg/L. But convincingly, 0.5 – 5.0 mg/L NAA with other PGR like 2,4-D would be more proper. Hence, extensive studies of these hormones and others will provide insights on their potentialities to embryogenic callus induction and probably identify more of their roles in PTC analysis.