T lymphocytes play positive and negative roles in the pathogenesis of allergic disease. Isolation and functional characterization of T lymphocyte subpopulations is an important aspect of understanding allergy models and allergy therapies. Measurement of the T cell surface proteins and T cell proliferation can provide insight into T cell activation. T cell function and the identities of T cell subsets can be determined by measuring cytokine production, either via intracellular cytokine staining or ELISPOT. This chapter outlines protocols for T cell isolation as well as the evaluation of surface protein expression, proliferation, intracellular cytokine staining, and ELISPOT. © 2013 Springer Science+Business Media, LLC.
CITATION STYLE
Barker, B. R. (2013). Evaluation of T cell function in allergic disease. Methods in Molecular Biology, 1032, 31–44. https://doi.org/10.1007/978-1-62703-496-8_3
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