Ws-9659 A and b, novel testosterone 5α-reductase inhibitors isolated from a streptomyces. I. Taxonomy, fermentation, isolation, physico-chemical characteristics

Abstract

WS-9659 A and B, produced by Streptomyces sp. No. 9659, were extracted from cultured broth, purified by solvent extraction followed by chromatography on silica gel and then isolated as prisms (C22H24N2O, mp 161-162°C, C22H23N2OCl, mp 152∼153°C). WS-9659 A and B have testosterone 5α-reductase inhibitory activity. The IC50 values of WS-9659 A and B for partially purified rat prostate testosterone 5α-reductase were 5.0 × 10-7 M and l.0× 10-5 M, respectively. © 1989, JAPAN ANTIBIOTICS RESEARCH ASSOCIATION. All rights reserved.