How cells interact with the extracellular matrix (ECM) is important for a number of fundamental processes in cell biology. However, the ECM is highly complex and in order to simplify the matrix for cell biological studies, it has been modeled with self-assembled monolayers (SAMs) of alkanethiolates on gold substrates. In this chapter, we outline procedures to create dynamic surfaces by functionalizing SAMs. SAMs based on quinone, oxyamine, and alcohol-terminated thiols were used to immobilize cell adhesive peptides with spatial control. Cells were seeded to these surfaces to provide cell co-culture patterns suitable for biological studies.
CITATION STYLE
Westcott, N. P., & Yousaf, M. N. (2011). Fabrication of dynamic self-assembled monolayers for cell migration and adhesion studies. In Methods in Molecular Biology (Vol. 751, pp. 421–438). Humana Press Inc. https://doi.org/10.1007/978-1-61779-151-2_26
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