Heparin is a potent clinically used anticoagulant. It is a heterogeneous mixture of polymers that contain a variety of sulfation patterns. However, only 3- O sulfonated heparin pentasaccharide units have been proven to bind to antithrombin and elicit an anticoagulant response. Heparins with other sulfation patterns are able to bind to a variety of other proteins such as FGF, VEGF, and CXCL-3. By modulating heparin’s sulfation pattern, it is possible to generate polymers that can regulate biological processes beyond hemostasis. Here we describe a variety of simple chemical modification methods, N -acetylation, N -deacetylation, N -sulfation, O -sulfation, 2- O desulfation, and complete desulfation, to prepare heparin-like polymers with distinct sulfation patterns.
CITATION STYLE
Raman, K., Kuberan, B., & Arungundram, S. (2015). Chemical modification of heparin and heparosan. Methods in Molecular Biology, 1229, 31–36. https://doi.org/10.1007/978-1-4939-1714-3_4
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