The new world artemia species A. franciscana and A. persimilis are highly differentiated for chromosome size and heterochromatin content

Abstract

Chromosomal rearrangements have played a key role in the speciation of the New World sexual Artemia species (Crustacea, Anostraca) A. franciscana and A. persimilis. The species differ by a chromosome duplication (2n + 2 = 44 in A. persimilis vs 2n = 42 in A. franciscana), and a greater amount of heterochromatin (HCH) in A. franciscana. To investigate this difference in HCH, four parameters were compared for the first time in Artemia : 1) the absolute sizes of one A. persimilis and four A. franciscana karyotypes; 2) the relative lengths of all chromosome; 3) the number of heterochromatic bands and 4) the relative amounts of HCH per chromosome and its position. The two A. franciscana karyotypes with the largest HCH amount (26%), have twice (139.26 μm and 134.05 μm) the absolute size of the A. persimilis karyotype (64.91 μm; HCH: 1.97%). Interspecific and intraspecific (A. franciscana) differences in chromosome size and HCH were observed, although the two sets of information are not positively correlated. While A. persimilis shares plesiomorphic karyological traits with Old World species, A. franciscana has apomorphic features such as longer chromosomes and greater HCH content, mainly dispersed towards telomeres. The impacts of such chromosome rearrangements are discussed in relation to the wider geographic distribution, greater colonizing ability, and life history plasticity of A. franciscana. An additional, though preliminary, point of this paper is the observation that the female would be the heterogametic sex. © Journal compilation © 2009 Hereditas.