Antibiotic susceptibility pattern of bacterial isolates from wound infection in Chitwan Medical College Teaching Hospital, Chitwan , Nepal

Abstract

The distribution of various pathogens causing wound infection was evaluated in Bharatpur, Chitwan, Nepal. A total of 200 pus specimens were cultured, of which 75{%} showed bacterial growth. Ten different species of bacteria were isolated. Staphylococcus aureus was the most common organism [65.3{%}] followed by Klebsiella pneumoniae [8{%}], Escherichia coli [7.3{%}], Coagulase negative staphylococci [6{%}], Pseudomonas aeruginosa [5.3{%}], Enterococcus species [3.3{%}], Enterobacter species [2{%}], Acinetobacter species [1.3{%}], Proteus mirabilis [0.6{%}] and Proteus vulgaris [0.6]. The antibiotic susceptibility test of the bacterial isolate was performed by Kirby-Bauer disk diffusion method. Majority of the bacterial isolates were resistant to almost all the antimicrobials employed. Among all the bacterial isolates, Escherichia coli was found to be highly resistant to commonly used antibiotics. High rate of multiple antibiotic resistance was observed among both Gram positive and Gram negative bacterial species recovered.