PHYTOCHEMICAL ANALYSIS, PROTEIN CONTENT & ANTIMICROBIAL ACTIVITIES OF SELECTED SAMPLES OF GLYCINE MAX LINN

Abstract

Two seed samples of Glycine max Linn. (S1, S2) were purchased from two retail stores of local market. Non-sprouted and sprouted seed powder were extracted separately with methano l (100%, 50%) by cold maceration to obtain methanol ic and hydroalcoholic extract of Glycine max Sample 1 was designated as M ES1 and HES1 and sample 2 as MES2 and HES2 respecti vely. Phytochemical analysis indicated the presence of various phytocon stituents viz. phytosterols, flavonoids, phenolic c ompounds, tannins, carbohydrates, proteins, amino acids, fixed oils an d fats etc. Thin layer chromatography study on extr acts revealed the presence of a number of compounds. The protein content of these swere studied. The protein content of samples MES1, HES1, MES2 and HES2 with respect to BSA was found to be 90.6 2µg/m l, 82µg/ml, 94.5µg/ml and 79.1µg/ml respectively. T he highest among these were found to be in MES2. Sprouting enhanced the pr otein content of the two samples. The samples have shown antimicrobial activity at selected concentration and microbial strains (26 mm) for gram negative bacteria (27mm) for gram posi tive bacteria.