Transient expression in plants is a valuable tool for many aspects of functional genomics and promoter testing. It can be used both to over-express and to silence candidate genes. It is also scaleable and provides a viable alternative to microbial fermentation and animal cell culture for the production of recombinant proteins. It does not depend on chromosomal integration of heterologous DNA so is a relatively facile procedure and can lead to high levels of transgene expression. Recombinant DNA can be introduced into plant cells via physical methods, via Agrobacterium or via viral vectors. © 2009 Humana Press, a part of Springer Science+Business Media, LLC.
CITATION STYLE
Jones, H. D., Doherty, A., & Sparks, C. A. (2009). Transient transformation of plants. Methods in Molecular Biology, 513, 131–152. https://doi.org/10.1007/978-1-59745-427-8_8
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