HSV glycoproteins play important roles in the viral life cycle, particularly viral cell entry. Here we describe the protocol for expression, purification, and crystallization of full-length HSV-1 glycoprotein B. The protocol provides a framework for incorporating transmembrane domain-stabilizing amphipols into the crystallization setup and can be adapted to isolate other complete HSV glycoproteins.
CITATION STYLE
Cooper, R. S., & Heldwein, E. E. (2020). Expression, Purification, and Crystallization of Full-Length HSV-1 gB for Structure Determination. In Methods in Molecular Biology (Vol. 2060, pp. 395–407). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9814-2_24
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