Peroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that also comprises β, δ, and γ isoforms. PPARα is a ligand-activated transcription factor that plays an important role in the regulation of many genes involved in key metabolic processes. Today, PPARα has been cloned from mammalian, marsupial, and a number of marine species and its expression has been found to be relatively tissue- and species-specific. Here, we describe the methods for cloning of PPARα genes by RT-PCR and RACE approaches and related protocols for studying the expression of cloned PPARα cDNAs in mammalian cell systems. © 2013 Springer Science+Business Media New York.
CITATION STYLE
Ngo, S. N. T., & McKinnon, R. A. (2013). PPAR-alpha cloning, expression, and characterization. Methods in Molecular Biology, 952, 7–34. https://doi.org/10.1007/978-1-62703-155-4_2
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