Identification of endogenous substrates of orphan cytochrome P450 enzymes through the use of untargeted metabolomics approaches

Abstract

Metabolomics provides an invaluable means to interrogate the function of "orphan" enzymes, i.e., those whose endogenous substrates are not known. Here we describe a high-performance liquid chromatography- coupled mass spectrometry (HPLC-MS)-based metabolomics approach to identify an endogenous substrate of an orphan cytochrome P450. © Springer Science+Business Media New York 2013.