Over-expression is a valid functional genomics approach to characterise genes of unknown function on a genome-wide scale. Strains are engineered to over-express a specific gene and the resulting gain-of-function phenotype assessed. Here, we describe the strategy we are adopting to synthesise a Candida albicans ORFeome collection and the options available to create over-expressing strains from this collection.
CITATION STYLE
Cabral, V., Chauvel, M., Firon, A., Legrand, M., Nesseir, A., Bachellier-Bassi, S., … d’Enfert, C. (2012). Modular gene over-expression strategies for candida albicans. In Methods in Molecular Biology (Vol. 845, pp. 227–244). Humana Press Inc. https://doi.org/10.1007/978-1-61779-539-8_15
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