Allelic exchange of unmarked mutations in mycobacterium tuberculosis

Abstract

Mycobacterium tuberculosis has been studied since the 19th century, but genetic manipulation of this organism has only become possible within the last decade of the 20th century. One key methodology is the allelic exchange of unmarked, in-frame deletion mutations and point mutations using counter-selectable suicide plasmids. I describe below the challenges of allelic exchange in M. tuberculosis, the overall mechanism of a suicide vector system, and how it can be used for efficient allelic exchange in this formally intractable organism. © 2008 Humana Press Inc.