d-Amino acids have recently attracted much attention in various research fields including medical, clinical, and food industry due to their important biological functions that differ from l-amino acid. Most chiral amino acid separation techniques require complicated derivatization procedures in order to achieve the desirable chromatographic behavior and detectability. This chapter describes a highly sensitive analytical method for the enantioseparation of chiral amino acids without any derivatization process using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The method allows the simultaneous analysis of 18 d-amino acids with high sensitivity and reproducibility. Additionally, this chapter also focuses on the application of the method to real samples for the quantification of targeted amino acids.
CITATION STYLE
Nakano, Y., Taniguchi, M., Umakoshi, Y., Watai, D., & Fukusaki, E. (2019). High-Throughput LC-MS/MS Method for Chiral Amino Acid Analysis Without Derivatization. In Methods in Molecular Biology (Vol. 2030, pp. 253–261). Humana Press Inc. https://doi.org/10.1007/978-1-4939-9639-1_19
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