Isolation of nuclear and cytoplasmic proteins from DT40 cells.

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Abstract

Nuclear and cytoplasmic proteins from DT40 B cells are extracted by serial fractionation in this protocol. The protein extracts are suitable for the detection of DNA-protein interaction, protein-protein interaction, DNase I footprinting analysis, and related techniques. This is a general protocol that was adapted as shown here to our use due to the fragile nature of DT40 cells and their nuclei.

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Wang, Y. D., & Caldwell, R. B. (2006). Isolation of nuclear and cytoplasmic proteins from DT40 cells. Sub-Cellular Biochemistry, 40, 439–440. https://doi.org/10.1007/978-1-4020-4896-8_43

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