Objective: The selection of reference genes in sugarcane under Sugarcane mosaic virus (SCMV) infection has not been reported and is indispensable to get reliable reverse transcription quantitative PCR (RT-qPCR) results for validation of transcriptome analysis. In this regard, seven potential reference genes were tested by RT-qPCR and ranked according to their stability using BestKeeper, NormFinder and GeNorm algorithms, and RefFinder WEB-based software in an experiment performed with samples from two sugarcane cultivars contrasting for SCMV resistance, when mechanically inoculated with a severe SCMV strain and using mock inoculated plant controls. Results: The genes Uridylate kinase (UK) and Ubiquitin-conjugating enzyme 18 (UBC18) were the most stable according to GeNorm algorithm and the Pearson correlation coefficients with the BestKeeper index. On the other hand, ribosomal protein L35-4 (RPL1), Actin (ACT) and Ubiquitin1 (UBQ1) were the least stable genes for all algorithms tested.
CITATION STYLE
Da Silva, M. F., Gonçalves, M. C., Dos Santos Brito, M., Nóbile, P. M., De Andrade, L. M., Medeiros, C. N., … Pinto, L. R. (2019). Reference genes for gene expression studies targeting sugarcane infected with Sugarcane mosaic virus (SCMV). BMC Research Notes, 12(1). https://doi.org/10.1186/s13104-019-4168-5
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