Site-specific delivery of epigenetic modulating drugs into the rat brain

Abstract

Site-specific injection of epigenetic modulating drugs into the brain, using neurostereotaxic surgery, is a very valuable experimental tool. This technique enables the pharmacological manipulation of targeted molecules, such as DNMT and HDAC, in specific brain areas, in conscious animals. This chapter presents a step-by-step description of cannula implantation in targeted brain structures and microinjection using neurostereotaxic surgery.