Photolabile protection for one-pot sequential native chemical ligation

Abstract

Newly developed phenacyl-type protection (Mapoc) for amines provides a facile and efficient synthetic protocol for peptides/proteins, in which sequential native chemical ligation (NCL) in the same reaction vessel can be conducted in a purification-free manner. A synthetic strategy of sequential NCL followed by oxidation with DMSO for disulfidebond formation was successfully applied to the one-pot preparation of the human-brain natriuretic peptide (hBNP). © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.