The ease of use, robustness, cost-effectiveness, and posttranslational machinery make baculovirus expression system a popular choice for production of eukaryotic membrane proteins. This system can be readily adapted for high-throughput operations. This chapter outlines the techniques and procedures for cloning, transfection, small-scale production, and purification of membrane protein samples in a highthroughput manner.
CITATION STYLE
Kalathur, R. C., Panganiban, M., & Bruni, R. (2016). High-throughput baculovirus expression system for membrane protein production. In Methods in Molecular Biology (Vol. 1432, pp. 187–202). Humana Press Inc. https://doi.org/10.1007/978-1-4939-3637-3_12
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