Combinatorial one-bead-one-compound (OBOC) peptide library screening has proven to be a powerful tool for identification of small molecules, peptides, or peptidomimetics against a variety of specific targets such as cell surface receptors, protein kinases, proteases, and phosphatases. With each bead displaying many copies of a single chemical entity, millions of compounds can be rapidly synthesized and screened with whole-cell binding on-bead functional assays. Here we describe the methodology for the synthesis, screening, and sequence deconvolution of an OBOC peptide library analyzed for affinity to a cancer cell line.
CITATION STYLE
Bononi, F. C., & Luyt, L. G. (2015). Synthesis and cell-based screening of one-bead-one- compound peptide libraries. Methods in Molecular Biology, 1248, 223–237. https://doi.org/10.1007/978-1-4939-2020-4_15
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