Nanorobotic manipulation and sensing for biomedical applications

Abstract

We developed a method formanipulating a single virus (size: 100nm) that employs optical tweezers in conjunctionwith dielectrophoretic (DEP) concentration of viruses on a microfluidic chip. The DEP force in the sample chamber concentrates the virus and prevents it from adhering to the glass substrate. The concentrated virus is transported to the sample selection section where it is trapped by optical tweezers. We demonstrated concentration of the virus using the DEP force, transported a single virus, and made it contact to a specific H292 cell. We also developed local measurement with functional gel-microtool for cell measurement. Gel-microtool was impregnated with indicators. In this chapter, bromothymol blue (BTB) and bromocresol green (BCG) were employed as pH indicators. Rhodamine B is temperature-sensitive fluorescent dye and is used for temperature measurement. Gel-microtool is manipulated by optical tweezers. Measurement is performed by detecting the color and the fluorescence intensity of each gel-microtool. We succeeded in pH measurement and temperature measurement using the gel-microtools.