Biodegradation of textile wastewater by naturally attenuated enterobacter sp

Abstract

The exponential increase in anthropogenic activities has led to the accumulation of xenobiotics into the environment, synthetic dyes being one of the culprits. Noteworthy is the fact that the textile industry utilizes enormous volumes of water for dyeing and printing unit operations thereby generating wastewater proportionately. Taking into consideration, implications of toxic textile effluents, a pilot study was planned to screen for naturally attenuated bacterial isolates capable of degrading textile effluents. Requisite effluent samples were collected from Kelki Printers Co-operative Society Limited, Sanganer, Jaipur and bacterial screening was carried out by bioaccumulation of Remazol Brilliant Blue R (RBRR) (formation of halo around colonies). Of the 19 bacterial isolates obtained, the most promiscuous isolate was biochemically characterized as Enterobacter sp. For biodegradative investigations, it was inoculated in sterilized textile effluent and incubated at 37°C for 7 days under agitating conditions. Pre and post bacterial inoculation (1% v/v), Physico-chemical parameters were analysed following standard procedures. A significant (p<0.05) lowering of pollution indicators was monitored when contrasted with abiotic control. The present study was aimed to explore the role of naturally attenuated and effluent adapted Enterobacter sp. screened from untreated textile effluent based on its colour (RBRR) removal efficacy under in vitro conditions. Furthermore, it was also explored for its biodegradative properties to minimize the level of potential pollution indicators through the microcosm approach. This pilot study based on a three-tier approach encompassing bioprospecting, bio enrichment and bioaugmentation plausibly provided insights for enhanced degradation of real dye wastewaters by unlocking the biochemical pathways of adapted microbes.