The purification of native protein complexes requires the availability of sufficient amounts of starting material. Drosophila melanogaster embryos have proven to be a rich source for nuclear protein complexes. Here we describe establishment and maintenance of a fly facility for the production of large amounts of embryos, protocols for the production of nuclear extracts, and a scheme for the chromatographic purification of a nuclear multisubunit protein complex.
CITATION STYLE
Kunert, N., & Brehm, A. (2008). Mass production of Drosophila embryos and chromatographic purification of native protein complexes. Methods in Molecular Biology (Clifton, N.J.), 420, 359–371. https://doi.org/10.1007/978-1-59745-583-1_23
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