The CD8(+), CD8(-), and plasmacytoid dendritic cell (DC) subtypes develop from progenitors that express surface fms-like tyrosine kinase 3 (Flt3). Recently, two developmentally sequential progenitors have been identified that give rise to these subtypes. This includes a transition from an earlier CD11c(-)MHC-II(-) "pro-DC," which divides and differentiates to give rise to CD11c(+)MHC-II(-) "pre-DC," en route to generating the three CD11c(+)MHC-II(+) DC subtypes - plasmacytoid DCs, CD8(+) DCs, and CD8(-) DCs. In this chapter, we describe the very simple method of generating large numbers of in vitro-derived pro-DCs and pre-DCs. As these precursors are largely DC-restricted, they can be used to either reconstitute a mouse with DCs of desired background, to study the developmental steps in vitro or in vivo, among other purposes.
CITATION STYLE
Naik, S. H. (2010). Generation of large numbers of pro-DCs and pre-DCs in vitro. Methods in Molecular Biology (Clifton, N.J.), 595, 177–186. https://doi.org/10.1007/978-1-60761-421-0_11
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