Effect of Cinnamon Addition to an High-Sugar Meal on the Postprandial Blood Glucose Response of Healthy Subjects

Abstract

The mousse was subjected to a hydro-ethanolic extract (20:80). Posteriorly, the mixture was filtered using the Whatmanpaper filter. Homogeneous samples were obtained and subjected to chemical analysis. Total phenolic content determination The extracts total phenolic concentration was determined according Folin-Ciocalteu method [4] employing gallic acid as standard. The samples were analysed in triplicate: a volume of 312.5 μl were pipette to an ethanol: water 80: 20 (V/V) solution, to which was added 187.5 μl of water, 5 ml of solution Folin-Ciocalteu reagent (1:10 diluted with water) and 4 ml of aqueous solution Na 2 CO 3 1M. After 15 min the absorbance was measured at 765 nm. Gallic acid was used as the standard solution (Y=0.0034X+0.018 (R 2 =0.9966)) and the results were expressed in mg of gallic acid equivalents (GAE) / L. This method was adapted from the method described by [27] and is based on the activity of the antioxidants to reduce Fe 3+ , a colorless ferric complex to a blue-colored ferrous complex, Fe 2+ in the presence of 2,4,6-tri(2-piridil)-s-triazine (TPTZ). The FRAP solution was previously prepared, adding 25 ml of sodium acetate 300 mM pH 3.6,to2.5 ml of 10 mM TPTZ in HCl 40mM, and 2,5ml of FeCl 3 .6H 2 O 20 mM. 150 μl of the samples were added with 2850 μl of FRAP solution. The mixture was kept in the dark for 30 minutes and then the absorbance was read at 593 nm. A blank solution was prepared, in which the sample was replaced by 150 µl of water, in the same conditions. The Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), a vitamin E analogous, was used as standard (Y=2,17x10-3X+2,32x10-2 (R 2 =0,998)) and results were expressed as μM TE. Statistical analysis Statistical analysis was performed with SPSS for Windows (version v19.0; SPSS Institute, Chicago, IL). Data are presented as mean ± SEM. Mann-Whitney U test was used to assess the food intake in the day prior to the intervention between 2 groups. Repeated Measures ANOVA of mixed type was used to assess the postprandial blood glucose level at different times, between 2 groups. Independent sample t-test was used for blood glucose levels area under the curve (AUC) and overnight fasting statistical analysis. All statistical tests were performed at the 5% level of significance. Results Sample characterization General characteristics of sample regarding age, weight (Kg), body mass index (BMI), fat mass (FM), muscular mass (MM), waist to hip ratio (WHR) and waist circumference (WC) are represented in table 1. Total calories, macronutrient and dietary fiber intake at the day before the intervention were estimated for both groups. Statistical comparison with Mann-Whitney test revealed homogeneity between groups (p>0.05), table 2. ingested of 100g of mousse without cinnamon and in group B subjects ingested 100g of mousse with cinnamon. Blood samples were then collected, for each participant, at 30 (t 30), 60 (t 60), 90 (t 90) and 120 (t 120) minute after ingestion reference and test meal. Sterilized lancet, glucosemeter equipment and strips for glucosemeter (FreeStyle_Abbott Diabetes Care) were used for blood glucose level measurement. Blood glucose concentrations were measured with the OneTouch Vita® glucometer, which has an accuracy of 98%.