Directed differentiation is a powerful cell culture technique where developmental pathways are applied to a pluripotent progenitor in order to generate specific terminally differentiated cell populations. Here, we describe a serum-free protocol using growth factors in defined concentrations to derive iPSC-hepatic cells starting from both feeder and feeder-free conditions. The generated iPSC-hepatic cells are developmentally similar to fetal stage hepatocytes, and when generated from patients with genetic mutations such as alpha-1 antitrypsin deficiency recapitulate pathologic changes associated with clinical disease, such as protein misfolding, intracellular retention of misfolded proteins, and elevated levels of ER stress.
CITATION STYLE
Kaserman, J. E., & Wilson, A. A. (2017). Protocol for directed differentiation of human induced pluripotent stem cells (iPSCs) to a hepatic lineage. In Methods in Molecular Biology (Vol. 1639, pp. 151–160). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7163-3_15
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