Conformation sensitive gel electrophoresis (CSGE) is a rapid screening method for the detection of DNA sequence variation, specifically single-base changes or small insertions and deletions. It has been widely used for mutation screening in genetic disorders and for the detection of single nucleotide polymorphisms (SNPs). CSGE is a simple manual method, based on heteroduplex analysis, and compares well in terms of sensitivity with other screening technologies. CSGE also lends itself to automation and such modifications have been useful in increasing sample throughput and sensitivity. However, manual CSGE remains a low-cost, accessible, and effective approach for mutation screening, which can be carried out with minimal specialist equipment. This chapter describes manual CSGE, and outlines some of the uses, modifications, and limitations of this method.
CITATION STYLE
Hill, M. (2011). Conformation Sensitive Gel Electrophoresis. In Methods in Molecular Biology (Vol. 688, pp. 7–16). Humana Press Inc. https://doi.org/10.1007/978-1-60761-947-5_2
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