Effect of culture age on mepanipyrim-mediated inhibition of pectinase secretion in Botrytis cinerea

Abstract

The fungicide mepanipyrim tended to affect both mycelial growth and pectinase secretion of younger B. cinerea mycelia more strongly than older cultures, which is suggestive of its mode of action. Mepanipyrim was more effective in inhibiting pectinase secretion and in disease control activity against B. cinerea in the early stages of growth and infection. The uptake of mepanipyrim into the mycelia of B. cinerea was reduced as the culture aged and was inhibited by the mitochondrial uncoupling agent CCCP. This suggested that active energy metabolism results in greater mepanipyrim uptake, resulting in good inhibition of mycelial growth, pectinase secretion and disease control in younger B. cinerea. © Pesticide Science Society of Japan.