Raman spectroscopy detects melanoma and the tissue surrounding melanoma using tissue-engineered melanoma models

13Citations
Citations of this article
31Readers
Mendeley users who have this article in their library.

This article is free to access.

Abstract

Invasion of melanoma cells from the primary tumor involves interaction with adjacent tissues and extracellular matrix. The extent of this interaction is not fully understood. In this study Raman spectroscopy was applied to cryo-sections of established 3D models of melanoma in human skin. Principal component analysis was used to investigate differences between the tumor and normal tissue and between the peri-tumor area and the normal skin. Two human melanoma cells lines A375SM and C8161 were investigated and compared in 3D melanoma models. Changes were found in protein conformations and tryptophan configurations across the entire melanoma samples, in tyrosine orientation and in more fluid lipid packing only in tumor dense areas, and in increased glycogen content in the peri-tumor areas of melanoma. Raman spectroscopy revealed changes around the perimeter of a melanoma tumor as well as detecting differences between the tumor and the normal tissue.

Cite

CITATION STYLE

APA

Yorucu, C., Lau, K., Mittar, S., Green, N. H., Raza, A., Rehman, I. U., & MacNeil, S. (2016). Raman spectroscopy detects melanoma and the tissue surrounding melanoma using tissue-engineered melanoma models. Applied Spectroscopy Reviews, 51(4), 243–257. https://doi.org/10.1080/05704928.2015.1126840

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free