Integrative Omics Analysis Reveals a Limited Transcriptional Shock After Yeast Interspecies Hybridization

16Citations
Citations of this article
41Readers
Mendeley users who have this article in their library.

Abstract

The formation of interspecific hybrids results in the coexistence of two diverged genomes within the same nucleus. It has been hypothesized that negative epistatic interactions and regulatory interferences between the two sub-genomes may elicit a so-called genomic shock involving, among other alterations, broad transcriptional changes. To assess the magnitude of this shock in hybrid yeasts, we investigated the transcriptomic differences between a newly formed Saccharomyces cerevisiae × Saccharomyces uvarum diploid hybrid and its diploid parentals, which diverged ∼20 mya. RNA sequencing (RNA-Seq) based allele-specific expression (ASE) analysis indicated that gene expression changes in the hybrid genome are limited, with only ∼1–2% of genes significantly altering their expression with respect to a non-hybrid context. In comparison, a thermal shock altered six times more genes. Furthermore, differences in the expression between orthologous genes in the two parental species tended to be diminished for the corresponding homeologous genes in the hybrid. Finally, and consistent with the RNA-Seq results, we show a limited impact of hybridization on chromatin accessibility patterns, as assessed with assay for transposase-accessible chromatin using sequencing (ATAC-Seq). Overall, our results suggest a limited genomic shock in a newly formed yeast hybrid, which may explain the high frequency of successful hybridization in these organisms.

Cite

CITATION STYLE

APA

Hovhannisyan, H., Saus, E., Ksiezopolska, E., Hinks Roberts, A. J., Louis, E. J., & Gabaldón, T. (2020). Integrative Omics Analysis Reveals a Limited Transcriptional Shock After Yeast Interspecies Hybridization. Frontiers in Genetics, 11. https://doi.org/10.3389/fgene.2020.00404

Register to see more suggestions

Mendeley helps you to discover research relevant for your work.

Already have an account?

Save time finding and organizing research with Mendeley

Sign up for free