The generation of allergen-specific TCR transgenic animals allows for the characterization of allergen-specific T-cell responses in vivo and in vitro and is a powerful tool to study adaptive immunity to allergens. Here we describe an approach starting from the isolation of antigen-specific T-cell hybridomas and using PCR, flow cytometric, and co-culture methods to obtain antigen-specific MHC class II-restricted CD4 + TCR transgenic mice on the Rag2 −/− background.
CITATION STYLE
Vanheerswynghels, M., Toussaint, W., Schuijs, M., Vanhoutte, L., Killeen, N., Hammad, H., & Lambrecht, B. N. (2018). The generation and use of allergen-specific TCR transgenic animals. In Methods in Molecular Biology (Vol. 1799, pp. 183–210). Humana Press Inc. https://doi.org/10.1007/978-1-4939-7896-0_15
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