Effect of Annonaceous Sp. Ethanolic Extract on Transforming Growth Factor- Β (TGF- Β) Expression on MCF-7 Cells Line

Abstract

Aims: To analyze effect of the Annonaceous sp. ethanolic extract to Transforming Growth Factor- β (TGF- β) expressions on MCF-7 cells line. Methods: This experimental analytic study used Randomized Complete Block Design (RCBD) with three repetitions. The extract was dissolved in 1 ml of DMSO with a concentration of 0.1%. Furthermore, dilution was made with a dose of 0 µg / ml (K); 25 µg / ml (P1); 50 µg / ml (P2); 100 µg / ml (P3); 200 µg / ml (P4). MCF-7 cells line were cultured using RPMI 1640 Medium with 80 – 90% confluent. The ethanolic extract of Annonaceous sp. was exposed to MCF-7 cells for 48 hours. Analyze Transforming Growth Factor- β (TGF- β) level using ELISA Methods with λ=405 nm. Results: The results of the ELISA analysis show that the ethanolic extract of Annonaceous sp. has a potential effect of decreasing TGF- β expression on the sample with treatment on some concentration (p< 0,05). Conclusion: The ethanolic extract of Annonaceous sp. has shown the potential effect to decreased of TGF- β level on MCF-7 cells line Keywords: Annonaceous sp ethanolic extract, TGF- β, MCF-7 cells line, anti-cancer